Journal: Methods in Molecular Biology

Article Title: Biolistic DNA Delivery

doi: 10.1007/978-1-62703-110-3

Figure Lengend Snippet: Fig. 2. Modi fi cation of the spacer of Helios Gene Gun. ( a) Original standard spacer with a length of 2.8 cm, ( b) Modi fi ed spacer with a length of 1.3 cm.

Article Snippet: Bio-Rad Helios Gene Gun System with low-pressure regulator (see Note 1) including BioRad Helios Gene Gun System Prep Station assembled as per manufacturer’s instructions.

Techniques:

Journal: Methods in Molecular Biology

Article Title: Biolistic DNA Delivery

doi: 10.1007/978-1-62703-110-3

Figure Lengend Snippet: Fig. 1. ( a ) The Helios gene gun device is commercially available from Bio-Rad. ( b ) In vitro gene delivery into attached monolayer cells using the Helios gene gun.

Article Snippet: Bio-Rad Helios Gene Gun System with low-pressure regulator (see Note 1) including BioRad Helios Gene Gun System Prep Station assembled as per manufacturer’s instructions.

Techniques: In Vitro

Journal: Methods in Molecular Biology

Article Title: Biolistic DNA Delivery

doi: 10.1007/978-1-62703-110-3

Figure Lengend Snippet: Fig. 2. Evaluation of ef fi ciency of plasmid DNA delivery with the low pressure gene gun. ( a ) Empty plasmid vector (pGL3- Basic), 10 m g luciferase plasmid without particles and 1 m g luciferase plasmid coated on gold particles, respectively, was bombarded to C57BL/6 mice. After 48 h, luciferase activity was detected by in vivo image system. ( b ) Quanti fi cation of luciferase activity. 48 h after biolistic transfection, the skin from the plasmid-bombarded mice was homogenized. The luciferase activity of the skin lysate was determined.

Article Snippet: Bio-Rad Helios Gene Gun System with low-pressure regulator (see Note 1) including BioRad Helios Gene Gun System Prep Station assembled as per manufacturer’s instructions.

Techniques: Plasmid Preparation, Luciferase, Activity Assay, In Vivo, Transfection

Journal: Methods in Molecular Biology

Article Title: Biolistic DNA Delivery

doi: 10.1007/978-1-62703-110-3

Figure Lengend Snippet: Fig. 1. Schematic outline of the transiently transgenic mouse model. Mice are immunized transdermally with a codon optimized HCV NS3/4A DNA vaccine using the Helios Gene Gun device. Two weeks later the same mice receive a hydrodynamic injection (HDI) of 100 m g codon optimized HCV NS3/4A DNA to generate a transient hepatic expression of the transgene. At 72 h post-HDI the mice are sacri fi ced and liver specimen are analyzed for presence of HCV NS3-protein by immuno-precipitation and western blot (IP-WB) and by immunohistochemistry (IHC).

Article Snippet: Bio-Rad Helios Gene Gun System with low-pressure regulator (see Note 1) including BioRad Helios Gene Gun System Prep Station assembled as per manufacturer’s instructions.

Techniques: Transgenic Assay, Injection, Expressing, Immunoprecipitation, Western Blot, Immunohistochemistry

Journal: Methods in Molecular Biology

Article Title: Biolistic DNA Delivery

doi: 10.1007/978-1-62703-110-3

Figure Lengend Snippet: Fig. 2. Detection of transiently transgenic hepatocytes generated by a hydrodynamic injection of 100 m g of codon optimized HCV NS3/4A DNA plasmid ( a ) and ( b ). In vivo clearance of HCV NS3-protein (cells stained in brown ) in a mouse immunized once with 2 m g codon optimized HCV NS3/4A DNA plasmid using the Helios Gene Gun device 2 weeks prior the hydrody- namic injection ( a ) and in a non-immunized mouse ( b ). In ( c ) a naïve control mouse is shown. The immuno-histochemical detection of the HCV NS3-protein is visualized 72 h post the hydrodynamic injection. In ( d ) detection of HCV NS3-protein in liver homogenates from immunized and non-immunized mice using immuno-precipitation and western blot. The mouse Ig heavy chain (50 kDa) was used as a loading control.

Article Snippet: Bio-Rad Helios Gene Gun System with low-pressure regulator (see Note 1) including BioRad Helios Gene Gun System Prep Station assembled as per manufacturer’s instructions.

Techniques: Transgenic Assay, Generated, Injection, Plasmid Preparation, In Vivo, Staining, Control, Immunoprecipitation, Western Blot

Journal: Methods in Molecular Biology

Article Title: Biolistic DNA Delivery

doi: 10.1007/978-1-62703-110-3

Figure Lengend Snippet: Fig. 4. Schematic outline of the tumor mouse model. Mice are immunized transdermally with a codon optimized HCV NS3/4A DNA vaccine using the Helios Gene Gun device. Two weeks later the same mice are inoculated with 1 × 10 6 Sp2/0 cells expressing the HCV NS3/4A protein. Tumor sizes/volumes are measured during a 2–3 week period using a sliding caliper.

Article Snippet: Bio-Rad Helios Gene Gun System with low-pressure regulator (see Note 1) including BioRad Helios Gene Gun System Prep Station assembled as per manufacturer’s instructions.

Techniques: Expressing

Journal: Methods in Molecular Biology

Article Title: Biolistic DNA Delivery

doi: 10.1007/978-1-62703-110-3

Figure Lengend Snippet: Fig. 6. Illustrative pictures of BALB/c mice prior to shaving and immunization ( a ), after shaving and one immunization ( b ), and 2 weeks postimmunization ( c ). The red arrow in ( b ) shows the target area of the Helios Gene Gun immunization ( gold colored mark ). The arrows in ( c ) indicate increased fur growth at the place for delivery.

Article Snippet: Bio-Rad Helios Gene Gun System with low-pressure regulator (see Note 1) including BioRad Helios Gene Gun System Prep Station assembled as per manufacturer’s instructions.

Techniques: